酿酒葡萄皮渣对绵羊瘤胃代谢及发育的影响

doi:10.11843/j.issn.0366-6964.2017.09.013

Abstract

Abstract:

The objective of present study was to investigate the effect of dietary wine grape pomace (WGP) supplementation on metabolism and development of rumen in lambs. A total of 24 Dorper (♂)×Small Tail Han sheep (♀) F1 male lambs ((25±1) kg of body weight, 5 month old) were randomly selected and divided into 4 groups equally. Lambs in 4 groups were fed with diets supplemented with WGP at 0%, 5%, 10%, 20% on a dry weight basis (group 0%, group 5%, group 10%, group 20%), respectively. The experiment lasted for 80 d. At the end of experiment, all lambs were slaughtered, and stomaches were weighted. The rumen fluid was collected for analyzing rumen fermentation and enzymes activity of microorganism, and rumen tissues were sampled for epithelial development measurements. The results showed that:1) No significant effect of dietary WGP supplementation on rumen pH and total volatile fatty acid (TVFA) concentration was observed (P>0.05). Propionic acid molar ratio was significantly increased, whereas acetic acid molar ratio and acetic acid/propionic acid ratio were decreased when lambs were fed with WGP diet (P<0.05); meanwhile, decreased concentrations of lactic acid, ammonia nitrogen and urea nitrogen were observed (P<0.05). 2) Amylase, protease and cellulases activity (except for pectase) were decreased with the increasing WGP supplementation levels (P<0.05), while pectase activity was exhibited as group 10% > 0% > 5% > 20% (P<0.05), the total protein concentration in rumen fluid had no significant difference among different groups(P>0.05). 3) Among factors related to VFA absorption and metabolism, relative mRNA expression of MCT1 and AE2 were significantly decreased when lambs were fed with WGP diet (P<0.05), whereas no significant difference at MCT4 and Na⁺-K⁺-ATPase mRNA expression levels among different groups were observed (P>0.05). 4) Both absolute weight and relative weight of rumen were not significantly altered among the groups (P>0.05). The cuticle thickness of lambs in WGP-added groups was significantly higher than that in group 0% WGP, and ruminal papillae length in group 20% WGP was significantly lower than that in other groups (P<0.05). In conclusion, WGP could effectively change rumen metabolism, and benefit to rumen development. Moreover, WGP supplementation amount could not exceed 10% considering the rumen epithelial tissue development.

CLC Number:

S826.5

JIN Ya-qian, ZHAO Jun-xing, LIU Wen-zhong, REN You-she, ZHANG Chun-xiang, ZHANG Wen-jia, XIANG Bin-wei, ZHANG Jian-xin. Effect of Dietary Wine Grape Pomace Supplementation on Rumen Metabolism and Development in Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1683-1693.

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Effect of Dietary Wine Grape Pomace Supplementation on Rumen Metabolism and Development in Lambs

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